Source: The AGE modified BSA was produced by reacting BSA with glucose under sterile conditions for 2 months followed by dialysis and purification according to the method described in Valentia et al. (2004) Formulation: Endotoxin: Preservative: Sterility: QC tests: Storage and stability: Upon receiving, store the product at -20°C. After thawing, store the working aliquots at 2-8°C for up to 1 month. For extended storage, aliquot the solution and freeze at -70°C or -20°C. Avoid repeated freezing and thawing. Activity: Extent of AGE modification: 25-30 adducts per Glucose-AGE-BSA, as judged by free amine content analysis and MALDI-TOF analysis. About Glucose-AGE-BSA: Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for AGE (RAGE). The fluorescence of Glucose-AGE-BSA was determined by fluorescence spectrometry with Ex./Em.=360/465 nm. The Glucose-AGE-BSA shows a 100-fold increase in fluorescence when compared to the control BSA. Browning of Glucose-AGE-BSA was determined by measuring the absorbance at 320 nm. The Glucose-AGE-BSA shows a 40 fold increase in absorbance when compared to the control BSA. Reference:
