生物离子/内毒素去除Maxi试剂盒/1/ER-003
商品编号：
ER-003
品牌：
biopioneerinc
市场价：
¥4400.00
美元价：
2200.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Endotoxin Removal Maxi Kit
1. Add 20ml of maxi plasmidinto 20-mL Endotoxin removalBuffer in a 50-mL conical tube
2. Mix by inverting the tube a few times and put on ice for about 5-10 minutes until the solution is clear without turbidity;
3. Mix well again by inverting the tube a few times;
4. Incubate the tube at 37 ?C water bath for about 5-10 minutes and the solution shall be turbid or separated;
5. Centrifuge at 3,000 rpm in a low-speed centrifuge at room temperature for 5 minutes with the brake off;
6. Carefully transfer the upper clear layer solution to another 50-mL conical tube;
7. Precipitate plasmid DNA with 0.1 volume of 3 M NaAc (pH 5.4) and 2 volume of 100% ethanol.
Biopioneer Brand:
品牌介绍
BP-Fectin TM哺乳动物细胞转染试剂是阳离子聚合物的专有配方，为实现高转染效率，低细胞毒性和易用性而优化。BP-Fectin TM转染试剂可替代许多其他转染技术，包括磷酸钙共沉淀，电穿孔，显微注射，生物弹颗粒递送以及与DEAE-葡聚糖的复合物形成。BP-凝集素转染试剂在哺乳动物和昆虫细胞的稳定和瞬时转染中均具有出色的性能。1.5毫升毫升的BP-Fectin TM转染试剂可提供足够的试剂来进行多达400x1ug的转染。BP-Fectin TM 转染试剂以即用型无菌溶液形式提供，是多孔板形式的高通量转染的理想选择。
