2xHotStart Taq Master Mix is a ready-to-use mixture of HotStart Taq DNA Polymerase, PCR Buffer, and dNTPs. Room-temperature reaction setup, is fast and easy. Simply pipet 12.5ul HotStart Taq Master Mix into each PCR tube and add 12.5ul of primers and template DNA diluted in the distilled water.

HotStart Taq master mix contains recombinant Hotstart Taq DNA polymerase and is for routine primer extension. It is efficient for amplification of template up to 5kb. Each reaction contains 1U of HotStart Taq DNA polymerase, 1.5mM Mg++ and 400uM dNTPs.

Protocol:

The monoclonal antibody based HotStart requires no prolonged heating or denaturing step as do other HotStart methods.

For each reaction, take 12.5ul of the HotStart mastermix and add to it a total of 12.5ul of primer/template/ diH2O to make up a final reaction volume of 25ul.

See the details below:

For each reaction:

Components / Volume (ul)

Supermix / 12.5 ul

Primers, Template, H20 / 12.5 ul

Template / X ul

Primer 1 / X ul

Primer 2 / X ul

H2O / X ul

Total / 25 ul

Stability: It is stable for 8-10 months, if it is kept at –20C.

Note: Don’t vortex the supermix, gently mix the super mix with the template, primers and water by pipetting up and down.