Product DescriptionRecombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B ,accession number NM_000924).

FormatEach vial contains 1 X 10^6 cells in 1 ml of 10% DMSO.

IntroductionPDE1B plays a role in signal transduction by regulating the intracellular concentration ofcyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5"-monophosphate. Although it prefers cGMP at low substrate levels it has a Vmax that isapproximately equal for both cGMP and cAMP (Polli JW and Koncaid RL, 1994; Yu J.et al., 1997).

ReferencesPolli, JW and Kincaid RL. (1994). “ Expression of a calmodulin-dependentphosphodiesterase isoform (PDE1B1) correlates with brain regions having extensivedopaminergic innervation.” J. Neurosc. 14 : 1251-1261.

Yu, J, et al. (1997). “Identification and Characterisation of a Human Calmodulin-Stimulated Phosphodiesterase PDE1B1 “ Cell Signalling 9 (7): 519-529.

Bender A.T. et al., (2005). “ Selective up-regulation of PDE1B2 upon monocyte-tomacrophagedifferentiation.” PNAS 102 (2): 497-502 .

Wunder F et al., (2009). “A novel PDE2A reporter cell line: characterization of thecellular activity of PDE inhibitors.” Mol. Pharm. 6 (1): 326-36.