Product Description The NF-kBreporter(Luc)-HEK293celllineisdesignedfor monitoringnuclearfactor KappaB(NF-kB)signaltransductionpathways.Itcontainsafireflyluciferasegene driven byfourcopies ofthe NF-kBresponse elementlocated upstream ofthe minimal TATApromoter.Afteractivationbypro-inflammatorycytokinesorstimulantsof lymphokinereceptors,endogenousNF-kBtranscriptionfactorsbindtotheDNA response elements, inducing transcription of the luciferase reporter gene.Format Each vial contains 2 X 10^6 cells in 1 ml of 10% DMSO.

Mycoplasma Testing The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

References 1.Pessara U, Koch N (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-kB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.

2.Baeuerle PA (1998) Pro-inflammatory signaling: last pieces in the NF-kB puzzle? Curr Biol. 8(1):R19-R22.

3.Takada Y, Kobayashi Y, Aggarwal BB (2005) Evodiamine abolishes constitutive and inducible NF-kB activation by inhibiting IkBα kinase activation, thereby suppressing NF-kB-regulated antiapoptotic and metastatic gene expression, up-regulating apoptosis, and inhibiting invasion. J Biol Chem. 280(17):17203-17212.