生物离子/人重组LIF，10ug/1/SC-043-1
商品编号：
SC-043-1
品牌：
biopioneerinc
市场价：
¥4500.00
美元价：
2250.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DescriptionLeukemia Inhibitory Factor (LIF), a lymphoid factor, belongs to the interleukin 6 class cytokine1. LIF binds to the specific LIF receptor (LIFR-α) and forms a heterodimer with the GP130 signal transducing subunit, which leads to the activation of the JAK/STAT and MAPK cascades2. LIF is also involved in a wide variety of biological processes including hematopoietic cell proliferation, neuronal survival and formation, and bone formation. It is used to maintain the pluripotency of mouse embryonic stem cell (ESC). Using the current human ESC culture method, human LIF is not required. But recent studies showed that the human ESC is the prime stem cell. The more totipotent human ESC, or called naïve stem cell, which is more like mouse ESC, can be isolated using LIF or inducedby introducing Oct4, Sox2, Klf4, cMyc, with small molecules3,4 or plus two other factors Nr5a2, and RxRγ into the differentiated cells5.
.
Purity > 99% (by SDS Page)
Buffer Phosphate buffered saline with 1% w/v BSA.
Endotoxin Level < 1.0 EU/mg as determined by the LAL method
Biologic Activity The specific activity is at least 1 x 106 units/ml, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 myeloid leukemia cell colonies in 1 ml agar cultures.
Storage & HandlingStore at 4°C.Freeze-thaw should be avoided.
Stability Stable for up to 6 months from date of receipt when stored as directed.
References 1.Williams R.L., Hilton, D.J., Pease, S., Willson, T.A., Stewart, C.L., et al (1988)
Myeloid leukemia inhibitory factor maintains the developmental potential of
embryonic stem cells. Nature 336:686-7.
2. Marina Trouillas, Claire Saucourt, Bertrand Guillotin, Xavier Gauthereau, Jean-Luc Taupin, Jean-François Moreau, Hélène Bœuf (2009) European Cytokine Network 20:51-62.
3. Hanna, J., Cheng, A.W., Saha, K., Kim, J., Lengner, C.J., Soldner, F., Cassady, J.P., Muffat, J., Carey, B.W., and Jaenisch, R. (2010) Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs. Proc. Natl. Acad. Sci. USA 107: 9222-9227.
4. Xu Y, Zhu X, Hahm HS, Wei W, Hao E, Hayek A, Ding S (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. PNAS 107:8129-34.
5. Wang W, Yang J, Liu H, Lu D, Chen X, Zenonos Z, Campos LS, Rad R, Guo G, Zhang S, Bradley A, Liu P. (2011) Rapid and efficient reprogramming of somatic cells to induced pluripotent stem cells by retinoic acid receptor gamma and liver receptor homolog 1. PNAS 108:18283-8.
Biopioneer Brand:
品牌介绍
BP-Fectin TM哺乳动物细胞转染试剂是阳离子聚合物的专有配方，为实现高转染效率，低细胞毒性和易用性而优化。BP-Fectin TM转染试剂可替代许多其他转染技术，包括磷酸钙共沉淀，电穿孔，显微注射，生物弹颗粒递送以及与DEAE-葡聚糖的复合物形成。BP-凝集素转染试剂在哺乳动物和昆虫细胞的稳定和瞬时转染中均具有出色的性能。1.5毫升毫升的BP-Fectin TM转染试剂可提供足够的试剂来进行多达400x1ug的转染。BP-Fectin TM 转染试剂以即用型无菌溶液形式提供，是多孔板形式的高通量转染的理想选择。
