Biotinylated Recombinant Human ACE2-His-Avi Protein (rhACE2-His)

Source: Biotinylated recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: Q9BYF1Predicted molecular mass: 86.5 kDa. Due to glycosylation, the biotinylated recombinant human ACE2 protein migrates to 95-110 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Activity: Immobilized recombinant SARS-CoV-2 (2019-nCoV) Spike S RBD at 1 ug/ml (100 ul/well). Dose response curve for the biotinylated recombinant human ACE2 protein with the EC50 of 0.1 ug/ml determined by ELISA. Conditions of optimal biotinylated recombinant Human ACE2 protein (rhACE2-His, rhACE2-Fc, and rcACE2-His) performance should be determined experimentally by the investigator.Formulation: The biotinylated recombinant human ACE2-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Background

ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.