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商品详细His-Spike/CVRP-0012重组生物亲和素
His-Spike/CVRP-0012重组生物亲和素
His-Spike/CVRP-0012重组生物亲和素
商品编号: CVRP-0010
品牌: biopioneerinc
市场价: ¥8700.00
美元价: 4350.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: sack
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi

Source: Biotinylated recombinant SARS-CoV-2 Spike RBD (Arg319-Asn532) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: QHD43416.1Predicted molecular mass: 27 kDa. Due to glycosylation, the recombinant SARS-CoV-2 Spike RBD migrates to 36-40 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike RBD-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 100 ng/ml determined by ELISA.Formulation: The biotinylated recombinant SARS-CoV-2 Spike RBD-His-Avi was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Background

The receptor binding domain (RBD) in the S1 subunit of the SARS-CoV-2 spike (S) glycoprotein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of virus entry inhibitors, neutralizing antibodies, and vaccines. There are two subunits, S1 and S2, in the SARS-CoV-2 spike (S) glycoprotein. The S1 subunit, the N-terminal 14–685 amino acids of S protein, contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM). The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD), and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV.

Both His-tagged and Fc-fused recombinant SARS-CoV-2 S1 RBD are available with an Avi site, which is for biotinylation. Please ask for a quote if you need protein mutants or a protein with different modification or components.

品牌介绍
BP-Fectin TM哺乳动物细胞转染试剂是阳离子聚合物的专有配方,为实现高转染效率,低细胞毒性和易用性而优化。BP-Fectin TM转染试剂可替代许多其他转染技术,包括磷酸钙共沉淀,电穿孔,显微注射,生物弹颗粒递送以及与DEAE-葡聚糖的复合物形成。BP-凝集素转染试剂在哺乳动物和昆虫细胞的稳定和瞬时转染中均具有出色的性能。1.5毫升毫升的BP-Fectin TM转染试剂可提供足够的试剂来进行多达400x1ug的转染。BP-Fectin TM 转染试剂以即用型无菌溶液形式提供,是多孔板形式的高通量转染的理想选择。