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当前位置: 首页 > 产品中心 > Virus_purification_kit > 生物离子/生物素化重组SARS-CoV-2 Spike S1 His-Avi蛋白,50ug/1/CVRP-0014
商品详细生物离子/生物素化重组SARS-CoV-2 Spike S1 His-Avi蛋白,50ug/1/CVRP-0014
生物离子/生物素化重组SARS-CoV-2 Spike S1 His-Avi蛋白,50ug/1/CVRP-0014
生物离子/生物素化重组SARS-CoV-2 Spike S1 His-Avi蛋白,50ug/1/CVRP-0014
商品编号: CVRP-0014
品牌: biopioneerinc
市场价: ¥9800.00
美元价: 4900.00
产地: 美国(厂家直采)
公司:
产品分类: 病毒纯化试剂盒
公司分类: Virus_purification_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein

Source: Biotinylated recombinant SARS-CoV-2 Spike S1 protein (Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.Accession: QHD43416.1Predicted molecular mass: 77.9 kDa. Due to glycosylation, the biotinylated recombinant SARS-CoV-2 Spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 1 EU per ug by the LAL method.Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.Formulation: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

品牌介绍
BP-Fectin TM哺乳动物细胞转染试剂是阳离子聚合物的专有配方,为实现高转染效率,低细胞毒性和易用性而优化。BP-Fectin TM转染试剂可替代许多其他转染技术,包括磷酸钙共沉淀,电穿孔,显微注射,生物弹颗粒递送以及与DEAE-葡聚糖的复合物形成。BP-凝集素转染试剂在哺乳动物和昆虫细胞的稳定和瞬时转染中均具有出色的性能。1.5毫升毫升的BP-Fectin TM转染试剂可提供足够的试剂来进行多达400x1ug的转染。BP-Fectin TM 转染试剂以即用型无菌溶液形式提供,是多孔板形式的高通量转染的理想选择。